We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues.
- Authors
Limborska, Svetlana; Dergunova, Lyudmila; Sudarkina, Olga; Filippenkov, Ivan; Brodsky, Ilya
- Abstract
Sphingomyelin synthase 1 (SMS1) catalyses the biosynthesis of sphingomyelin in eukaryotic cells. We have previously determined the structure of the SGMS1 gene encoding this enzyme and a number of its alternative transcripts. Here, we describe a study of the expression of the full-length SMS1 protein and the sum of the alternative transcripts encoding this protein in human tissues. The SMS1 protein and mRNA levels in tissues differed significantly and were not correlated, implying the active post-transcriptional regulation of SMS1 protein expression. The putative truncated isoforms of the SMS1 protein, which are encoded by a number of alternative transcripts, were not detected by immunoblotting and thus may be absent or present in only small amounts.
- Subjects
SPHINGOMYELIN; SYNTHASES; GENE expression; TRANSCRIPTION factors; IMMUNOBLOTTING; POLYMERASE chain reaction
- Publication
Molecular & Cellular Biochemistry, 2015, Vol 406, Issue 1/2, p91
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-015-2427-x