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- Title
Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed.
- Authors
Miyanoshita, Mitsuru; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto
- Abstract
Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.
- Subjects
RIBONUCLEASE P; NUCLEIC acids; FLUORESCENCE resonance energy transfer
- Publication
Bioscience, Biotechnology & Biochemistry, 2015, Vol 79, Issue 12, p2014
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1058699