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- Title
Roseomonas chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from activated sludge.
- Authors
Chu, Cui-Wei; Chen, Qing; Wang, Cheng-Hong; Wang, Hong-Mei; Sun, Zhong-Guan; He, Qin; He, Jian; Gu, Jin-Gang
- Abstract
A Gram-negative, aerobic, short rod-shaped, pink-pigmented, non-motile bacterium, designated BUT-13, was isolated from activated sludge of an herbicide-manufacturing wastewater treatment facility in Jiangsu province, China. Growth was observed at 0-5.5 % NaCl, pH 6.0-9.0 and 12-37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BUT-13 is a member of the genus Roseomonas, and shows high sequence similarities to R. pecuniae N75 (98.0 %) and R. rosea 173-96 (97.5 %), and lower (<97 %) sequence similarities to all other Roseomonas species. Chemotaxonomic analysis revealed that strain BUT-13 possesses Q-10 as the predominant ubiquinone; summed feature 8 (C w7 c and/or C w6 c; 38.8 %), C (16.6 %), C (15.2 %), summed feature 3 (C ω6 c and/or C ω7; 7.9 %) and C w9 c (4.7 %) as the major fatty acids. The polar lipids were found to consist of two aminolipids, a glycolipid, a phospholipid, a phosphoglycolipid, phosphatidylcholine, phosphatidylethanolamine and diphosphatidylglycerol. Strain BUT-13 showed low DNA-DNA relatedness with R. pecuniae N75 (45.2 %) and R. rosea 173-96 (51.2 %). The DNA G+C content was determined to be 67.6 mol%. Based on the phylogenetic analysis, DNA-DNA hybridization and chemotaxonomic analysis, as well as biochemical characteristics, strain BUT-13 can be clearly distinguished from all currently recognised Roseomonas species and should be classified as a novel species of the genus Roseomonas, for which the name Roseomonas chloroacetimidivorans sp. nov. is proposed. The type strain is BUT-13 (CCTCC AB 2015299 = JCM 31050).
- Subjects
CHLOROACETAMIDES; DNA analysis; LIPID analysis; WASTEWATER treatment; SPECIES distribution
- Publication
Antonie van Leeuwenhoek, 2016, Vol 109, Issue 5, p611
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-016-0664-y