We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Abnormalities in esophageal smooth muscle induced by mutations in collagen XIX.
- Authors
Sato, Haruna; Jin, Kyoko; Yano, Shinji; Yasuda, Aiko; Adachi, Sawako; Yoshioka, Hidekatsu; Kitamura, Hirokazu; Sasaki, Takako
- Abstract
Collagen XIX is a nonfibrillar collagen that localizes in restricted tissues at very low amounts. A previous study on Col19a1 null mice revealed that collagen XIX is involved in esophageal muscle physiology and morphogenesis. Here, we use histological analysis to show that mice with a Col19a1 mutant lacking the NC3 domain and seven collagen triplets display abnormal transition of smooth to striated muscle in the abdominal segment of esophagus, and a widened esophagus with age. With two newly prepared antibodies, we analyzed the expression of collagen XIX in the mouse esophagus and show that collagen XIX colocalizes with α-smooth muscle actin. By immunoelectron microscopy, we confirmed the localization of collagen XIX in esophageal smooth muscle cells. Col19a1 mutant mice contained reduced levels of mutated Col19a1 mRNA. Interestingly, hepatocyte growth factor, which has an important role in esophageal striated muscle development, was reduced in the esophagus of the Col19a1 mutant mice. These findings suggest that collagen XIX may be critical for the function of esophageal smooth muscle cells as a scaffold for anteroposterior migration of esophagus-striated muscle cells.
- Subjects
SMOOTH muscle; HEPATOCYTE growth factor; COLLAGEN; IMMUNOELECTRON microscopy; STRIATED muscle; ESOPHAGUS; CYTOKINES; GENETIC mutation; IMMUNOGLOBULINS; MUSCLE proteins; CELL culture; ANIMAL experimentation; GENE expression; ELECTRON microscopy; CELLS; MESSENGER RNA; LIVER cells; HISTOLOGY; MICE
- Publication
Histochemistry & Cell Biology, 2022, Vol 157, Issue 2, p205
- ISSN
0948-6143
- Publication type
Article
- DOI
10.1007/s00418-021-02059-9