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- Title
Ethyl tert-butyl ether (ETBE) biodegradation by a syntrophic association of Rhodococcus sp. IFP 2042 and Bradyrhizobium sp. IFP 2049 isolated from a polluted aquifer.
- Authors
Digabel, Yoann; Demanèche, Sandrine; Benoit, Yves; Vogel, Timothy; Fayolle-Guichard, Françoise
- Abstract
Ethyl tert-butyl ether (ETBE) enrichment was obtained by adding contaminated groundwater to a mineral medium containing ETBE as the sole carbon and energy source. ETBE was completely degraded to biomass and CO with a transient production of tert-butanol (TBA) and a final biomass yield of 0.37 ± 0.08 mg biomass (dry weight).mg ETBE. Two bacterial strains, IFP 2042 and IFP 2049, were isolated from the enrichment, and their 16S rRNA genes ( rrs) were similar to Rhodococcus sp. (99 % similarity to Rhodococcus erythropolis) and Bradyrhizobium sp. (99 % similarity to Bradyrhizobium japonicum), respectively. Rhodococcus sp. IFP 2042 degraded ETBE to TBA, and Bradyrhizobium sp. IFP 2049 degraded TBA to biomass and CO. A mixed culture of IFP 2042 and IFP 2049 degraded ETBE to CO with a biomass yield similar to the original ETBE enrichment (0.31 ± 0.02 mg biomass.mg ETBE). Among the genes previously described to be involved in ETBE, MTBE, and TBA degradation, only alkB was detected in Rhodococcus sp. IFP 2042 by PCR, and none were detected in Bradyrhizobium sp. IFP 2049.
- Subjects
ETHYL tert-butyl ether; BIODEGRADATION; RHODOCOCCUS; BRADYRHIZOBIUM; AQUIFER pollution; GROUNDWATER pollution
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 24, p10531
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-4803-3