We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences.
- Authors
Gab-Man Park
- Abstract
Abstract??To clarify the genetic relationships betweenClonorchis sinensisandOpisthorchis viverrini, patterns of inter-/intraspecific polymorphism were compared for four markers with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) in liver flukesC. sinensisfrom Korea (Kimhae) and China (Shenyang and Nanning) andO. viverrinifrom Laos (Savannakhet). Intra- and interspecific variations in the 18S, ITS2, and 28S rDNA and mitochondrial cytochromecoxidase subunit I (mtCOI) of mtDNA gene sequences were low and nearly identical. Three isolates ofC. sinensisshowed a high similarity (99?100%). No variation was detectable in the ITS2 sequence for theC. sinensisfrom Korea and China. ITS2 region sequences ofO. viverrinivsC. sinensisshowed 95% identity and differed at 28 nucleotide positions. Pairwise sequence divergence with threeC. sinensisisolates andO. viverriniranged from 0 to 3.94% in mtCOI gene. The mtCOI sequences were more highly conserved relative to the ITS2 sequences. These genetic data from different geographical areas showed that the liver flukes are not variable and are virtually identical almost despite belonging to entirely different genera.
- Subjects
LIVER flukes; GENETICS; GENETIC polymorphisms; PARASITES
- Publication
Parasitology Research, 2007, Vol 100, Issue 2, p351
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0269-x