We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Induction of Alternative Splicing and Inhibition of Activity of Telomerase Catalytic Subunit by Apoptotic Endonuclease EndoG in Human T, B, and NK Cells.
- Authors
Zhdanov, D. D.; Gladilina, Yu. A.; Pokrovskaya, M. V.; Aleksandrova, S. S.; Grishin, D. V.; Podobed, O. V.; Sokolov, N. N.
- Abstract
The effect of apoptotic endonuclease EndoG on alternative splicing of mRNA of human telomerase catalytic subunit hTERT (human telomerase reverse transcriptase) and telomerase activity in normal human lymphocytes were studied. Human CD4+, CD8+, B, and NK cells were transfected with a plasmid pEndoG-GFP containing <italic>EndoG</italic> gene or control plasmid pGFP. The levels of mRNA of <italic>EndoG</italic> or <italic>hTERT</italic> splicing variants were analyzed by real-time PCR. Protein content was assessed by Western blotting. Telomerase activity was measured by the telomere repeats amplification protocol. <italic>EndoG</italic> overexpression reduced the expression of active full-length <italic>hTERT</italic> and increased the expression of inactive splice variant. Shifted balance of hTERT splice variants in cells led to a significant decrease in telomerase activity within 72 h after transfection.
- Subjects
LYMPHOCYTES; TELOMERASE; APOPTOTIC bodies; ENDONUCLEASE structure; GENE transfection
- Publication
Bulletin of Experimental Biology & Medicine, 2018, Vol 164, Issue 4, p478
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-018-4016-y