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- Title
基于网络药理学的脉络舒通丸治疗痔疮作用机制研究.
- Authors
翟弋焱; 陈美琳; 黄佳奇; 时 锐; 伍 超; 张景媛; 张繁芹; 王 伟; 程国良; 李 冰; 谭影影; 黄志 鸿; 巫志姗; 吴嘉瑞
- Abstract
OBJECTIVE: To probe into the potential active components and possible mechanism of Mailuo Shutong pills in the treatment of hemorrhoids. METHODS: Chemical constituents of Mailuo Shutong pills and the related targets of hemorrhoids were obtained by Traditional Chinese medicine systems pharmacology database and analysis platform, DisGeNET, GeneCards and other databases. The intersection targets were imported into STRING database for analysis, and the analysis results were imported into Cytoscape software to construct protein-protein interaction (PPI) network diagram, Mailuo Shutong pills chemical components-target network diagram and drug-component-target-pathway network. The key targets were analyzed by gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis by R language. AutoDockTools software was used to verify the molecular docking between the active components of Mailuo Shutong pills and the corresponding key targets. RESULTS: A total of 215 compounds, 310 related targets, and 148 compound and disease intersection targets were screened. Key targets including protein kinase B (Akt) 1, tumor protein p53 (TP53), interleukin (IL) 6, epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA), ESR1, HIF1A and tumor necrosis factor (TNF) were identified. Based on the analysis of PPI network, there were 161 KEGG pathways and 3 041 GO functional entries were obtained. In the GO functional items, there were 2 706 biological process related items, 140 cellular components, and 195 molecular function related items. The results of molecular docking showed that quercetin, formononetin, luteolin, wogonin, kaempferol and other compounds had good binding activity with Akt1, TNF, TP53, IL-6, EGFR, VEGFA and ESR1. CONCLUSIONS: Mailuo Shutong pills in the treatment of hemorrhoids is based on the mechanism of multi-components and multi-targets. This study provides theoretical basis for further verification of related mechanisms.
- Subjects
PROTEIN kinase B; EPIDERMAL growth factor receptors; VASCULAR endothelial growth factors; TUMOR proteins; TUMOR necrosis factors; SIGNALS &; signaling; COMPOUND words
- Publication
Evaluation & Analysis of Drug-Use in Hospitals of China, 2022, Vol 22, Issue 12, p1439
- ISSN
1672-2124
- Publication type
Article
- DOI
10.14009/j.issn.1672-2124.2022.12.003