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- Title
Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system.
- Authors
Chen, Juan; Liu, Wu-Yu; Zhang, Xin; Pan, Qing; Wang, Feng-Shuya; Wen, Shi-Ming; Zhang, Jian-Hua; Wei, Ge-Hong
- Abstract
Aim : Hydrogen sulphide (H2S), as a signalling molecule, regulates the process of plant growth and development. H2S is involved in the establishment of the legume and rhizobial symbiotic relationship, but little is known about the role of H2S during nodule senescence. Method: We studied the function of H2S during nodule senescence in the Glycine max-Sinorhizobium fredii symbiotic system. Physiological and biochemical methods were used to modulate nitrogen fixation, nodule structure, senescence-related protein and gene expression. Results: Our results indicated that H2S decreased the senescent nodule number, increased nitrogenase (Nase) activity and ureide content, and further promoted the accumulation of NO3−-N, NH4+-N, and total N, which caused the accumulation of biomass in the late steps of symbiosis. H2S alleviated nodule senescence by maintaining the normal nitrogen fixation structure of nodules. Meanwhile, the expression levels of the NifH and NodGS proteins and the N metabolic enzyme activities were enhanced by H2S during nodule senescence. Moreover, H2S stimulated the expression of nitrogen metabolism-related genes in the nodules. Finally, senescence-related gene expression levels were regulated by H2S during nodule senescence. Conclusion: These findings suggested that H2S prolonged the lifespan of N2 fixation by delaying nodule senescence, which will be important for increasing soybean yield and agricultural sustainability development.
- Subjects
HYDROGEN sulfide; NITROGEN fixation; GLYCINE; GENE expression; LEGUMES; AGRICULTURAL development
- Publication
Plant & Soil, 2023, Vol 488, Issue 1/2, p603
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-023-05997-6