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- Title
Peptide-like substances as antimicrobial barriers to Corynebacterium sp. adhesion to silicone catheters.
- Authors
Fontana, M. B. C.; Bastos, M. C. F.; Brandelli, A.
- Abstract
Aims: To show medical application of antimicrobial peptides such as Pep5 and epidermin in inhibiting adhesion of Corynebacterium spp. to silicone catheters. Methods and Results: The inhibitory activity of crude preparations of Pep5 and epidermin was tested on Corynebacterium spp. isolated from catheter-related infections. The addition of these substances at 640 AU ml−1 to a cell suspension of Corynebacterium sp. 633544 resulted in a decrease of 3 log cycles in the number of viable cells over a period of 12 h. When Pep5 and epidermin were added to in vitro catheter colonization experiments, there was a decrease of 1 log unit ( P < 0·01) in the cell number of Corynebacterium spp. adhered to silicone catheters. Scanning electron microscopy revealed that antimicrobial-treated catheters presented zones with absence of adhered cells, and some parts of the catheter presented aggregates suggesting damaged cells. Conclusions: The crude preparations of Pep5 and epidermin were able to inhibit Corynebacterium sp. 633544 isolated from catheter-related infection. The capability of Pep5 and epidermin to inhibit catheter colonization may indicate their usefulness as a barrier to block or to reduce the bacteremia by Corynebacterium spp. Significance and Impact of the Study: Peptide-like antimicrobial substances used to reduce bacterial attachment to medical devices may represent a novel strategy to control catheter-related infections.
- Subjects
PEPTIDE antibiotics; ANTI-infective agents; CORYNEBACTERIUM; CATHETERS; SILICONES in medicine; INFECTION; CELL suspensions; SCANNING electron microscopy; CELLS
- Publication
Letters in Applied Microbiology, 2007, Vol 45, Issue 3, p324
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2007.02193.x