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- Title
The Identification of Drought Tolerance Candidate Genes in Oryza sativa L. ssp. Japonica Seedlings through Genome-Wide Association Study and Linkage Mapping.
- Authors
Liu, Tao; Li, Shuangshuang; Du, Haoqiang; Cui, Jingnan; Xu, Shanbin; Wang, Jingguo; Liu, Hualong; Zou, Detang; Lu, Wenhe; Zheng, Hongliang
- Abstract
Drought stress poses a significant threat to rice production, necessitating the identification of genes associated with drought tolerance. This study employed a combination of genome-wide association study (GWAS) and linkage mapping to pinpoint seedling drought tolerance genes in Japonica rice. Using the leaf rolling scale (LRS) as the phenotypic index, we assessed rice drought tolerance under polyethylene glycol-induced drought during the seedling stage. A lead SNP C8_28933410 by GWAS was identified, which was located within qLRS-8-1 identified by linkage mapping on chromosome 8. Combing the LD block analyses and QTL interval, a 138.6 kb overlap interval was considered as the candidate region. Haplotype analysis, qRT-PCR, sequence analysis, and mutant phenotype verification led to the speculation that LOC_Os08g05520 is a candidate gene associated with drought tolerance. Our findings provide a valuable reference for breeders aiming to enhance rice drought tolerance.
- Subjects
GENOME-wide association studies; DROUGHT tolerance; GENE mapping; RICE; SEEDLINGS; GENES
- Publication
Agriculture; Basel, 2024, Vol 14, Issue 4, p603
- ISSN
2077-0472
- Publication type
Article
- DOI
10.3390/agriculture14040603