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- Title
基于 X 型结构的 DNA 荧光传感器检测黄曲霉毒 素 B<sub>1</sub>.
- Authors
乔梦想; 白 天; 吕泽平; 卫 敏
- Abstract
Aflatoxin B1(AFB1 ) is a typical fungal toxin residual in agricultural products and poses a great threat to both humans and animals when it accumulates to a certain amount, therefore, accurate and rapid determination of AFB1 is of great importance for food safety. In this study, an X structure based on DNA nanotechnology was designed and a fluorescent aptamer sensor for the highly sensitive detection of AFB1 was constructed by combining magnetic separation technology. In this system, two DNA strands were firstly used to form an X-skeleton and immobilized on magnetic beads, and a fluorescent group-labeled aptamer strand was used as the signal probe ( FAM-Apt). When AFB1 is not present, FAM-Apt can form a double-stranded structure by base complementary pairing with the four ends of the X-skeleton, followed by magnetic separation and aspiration of the supernatant for fluorescence detection to obtain a low background signal. When AFB1 is present, FAM-Apt preferentially binds to AFB1 due to the high specificity and affinity of the target and the aptamer, and is detached from the sensor and free in the supernatant, which is detected after magnetic separation to obtain a higher fluorescence signal. Finally, the rapid detection of AFB1 was achieved according to the change of fluorescence signal intensity in the supernatant. Under the optimal experimental conditions, the relative fluorescence intensity of the sensor was linearly correlated with lg ( ρAFB1 ) in the range of 0. 05-100 ng / mL ( R² = 0. 99), and the detection limit was as low as 9 pg / mL. Satisfactory results were obtained by applying the designed sensor platform to spiked samples for recovery testing.
- Subjects
MYCOTOXINS; MAGNETIC separation; SEPARATION (Technology); DNA structure; BASE pairs; AFLATOXINS; DNA nanotechnology; DNA folding
- Publication
Journal of Henan University of Technology Natural Science Edition, 2023, Vol 44, Issue 5, p100
- ISSN
1673-2383
- Publication type
Article
- DOI
10.16433/j.1673-2383.2023.05.013