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- Title
Guanidino compounds inhibit acetylcholinesterase and butyrylcholinesterase activities: Effect neuroprotector of vitamins E plus C
- Authors
Delwing-de Lima, Daniela; Wollinger, Luiz Felipe; Casagrande, Ana Carolina Mesquita; Delwing, Fábio; da Cruz, José Geraldo Pereira; Wyse, Angela T.S.; Delwing-Dal Magro, Débora
- Abstract
Abstract: Hyperargininemia is a metabolic disorder biochemically characterized by tissue accumulating of arginine and other guanidino compounds. Convulsions, lethargy and psychomotor delay or cognitive deterioration are predominant clinical features of this disease. Although neurologic symptoms predominate in this disorder, their pathophysiology is still unknown. In the present study we initially investigated the in vitro effect of arginine, homoarginine, N-acetylarginine and argininic acid on acetylcholinesterase and butyrylcholinesterase in hippocampus and serum of 15-, 30- and 60-day-old rats. Results showed that arginine in vitro significantly decreased acetylcholinesterase activity in hippocampus of 15-day-old rats and increased this enzyme activity in hippocampus of 60-day-old rats, homoarginine and N-acetylarginine significantly increased acetylcholinesterase activity both in hippocampus of 15- and 30-day-old rats. On the other hand, butyrylcholinesterase was inhibited by homoarginine in serum of 15-day-old rats. The influence of the antioxidants trolox and ascorbic acid on the effects elicited by arginine, homoarginine and N-acetylarginine was also studied. Results showed that these antioxidants were able to prevent the alteration on acetylcholinesterase and butyrylcholinesterase activities caused by guanidine compounds studied, suggesting that alterations on these cholinesterases were probably mediated by free radicals. It is presumed that these results might be associated, at least in part, with the neuronal dysfunction of patients affected by hyperargininemia.
- Subjects
GUANIDINES; ACETYLCHOLINESTERASE; BUTYRYLCHOLINESTERASE; VITAMINS; METABOLIC disorders; ARGININE; OXIDATIVE stress; ENZYME inhibitors
- Publication
International Journal of Developmental Neuroscience, 2010, Vol 28, Issue 6, p465
- ISSN
0736-5748
- Publication type
Article
- DOI
10.1016/j.ijdevneu.2010.06.008