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- Title
Near-infrared photoactivatable control of Ca<sup>2+</sup> signaling and optogenetic immunomodulation.
- Authors
Lian He; Yuanwei Zhang; Guolin Ma; Peng Tan; Zhanjun Li; Shengbing Zang; Xiang Wu; Ji Jing; Shaohai Fang; Lijuan Zhou; Youjun Wang; Yun Huang; Hogan, Patrick G.; Gang Han; Yubin Zhou
- Abstract
The application of current channelrhodopsin-based optogenetic tools is limited by the lack of strict ion selectivity and the inability to extend the spectra sensitivity into the near-infrared (NIR) tissue transmissible range. Here we present an NIR-stimulable optogenetic platform (termed ’Opto-CRAC’) that selectively and remotely controls Ca2+ oscillations and Ca2+-responsive gene expression to regulate the function of non-excitable cells, including T lymphocytes, macrophages and dendritic cells. When coupled to upconversion nanoparticles, the optogenetic operation window is shifted from the visible range to NIR wavelengths to enable wireless photoactivation of Ca2+-dependent signaling and optogenetic modulation of immunoinflammatory responses. In a mouse model of melanoma by using ovalbumin as surrogate tumor antigen, Opto-CRAC has been shown to act as a genetically-encoded ’photoactivatable adjuvant’ to improve antigen-specific immune responses to specifically destruct tumor cells. Our study represents a solid step forward towards the goal of achieving remote and wireless control of Ca2+-modulated activities with tailored function.
- Subjects
OPTOGENETICS; IMMUNOREGULATION; PHOTOACTIVATION; RHODOPSIN; GENE expression; NEAR infrared radiation
- Publication
eLife, 2015, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.10056