We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bivariate cytofluorimetric analysis of nuclear protein and DNA relative to cell kinetics during germination of Pisum sativum seed.
- Authors
Sgorbati, Sergio; Sparvoli, Elio; Levi, Marisa; Chiatante, Donato
- Abstract
By means of a biparametric cytofluorimetric analysis it is possible to distribute meristematic plant cells in a variety of cell cycle sub-compartments, unidentifiable by DNA measurements alone. In this work, an asynchronous proliferating cell population of pea root meristems was divided into different sub-compartments of the cell cycle phases. i.e. G1A, G1B, S. G2A and G2B on the basis of their DNA-nuclear protein content. By means of the same biparametric analysis, differentiated mesophyll cells and quiescent cells of embryo roots, indicated as G0 and G2Q, were distinguished from cycling cells by their low nuclear protein content. These results conform to those of some analyses performed on animal cells in culture and show that it is possible to get a major insight into cell cycle kinetics and its control in a natural system such as root meristem.
- Subjects
PLANT roots; PEAS; FLUORIMETRY; CELLS; GERMINATION; PISUM
- Publication
Physiologia Plantarum, 1989, Vol 75, Issue 4, p479
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1989.tb05612.x