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- Title
Structural and functional microvascular alterations in a rat model of metabolic syndrome induced by a high-fat diet.
- Authors
Nascimento, Alessandro R.; Machado, Marcus; Jesus, Nathália; Gomes, Fabiana; Lessa, Marcos A.; Bonomo, Isabela T.; Tibiriçá, Eduardo
- Abstract
Objective To investigate microvascular alterations in an experimental model of metabolic syndrome induced by a high-fat diet (HFD) associated with salt supplementation (0.5% NaCl). Design and Methods Wistar Kyoto rats were fed standard chow (control group, CONT) or HFD for 20 weeks. The functional capillary density (FCD) was assessed using intravital fluorescence videomicroscopy. Results The HFD group presented a higher systolic blood pressure, plasma glucose and insulin levels, total and LDL-cholesterol levels, triglycerides, and visceral and epididymal fat when compared with the CONT group. When compared with the CONT group, the HFD group showed a lower FCD in the skeletal muscle ( P < 0.05) but not in the skin ( P > 0.05). The HFD group also had a lower capillary-to-fiber ratio in the skeletal muscle ( P < 0.01). The capillary volume density-to-fiber volume density ratio in the left ventricle of the HFD was also reduced ( P < 0.01). Finally, rats fed with HFD showed ventricular hypertrophy and increased cardiomyocyte diameter ( P < 0.01). Conclusions The long-term administration of a HFD associated with salt supplementation to rats generates an experimental model of metabolic syndrome characterized by central body fat deposition, insulin resistance, glucose intolerance, hypertriglyceridemia, hypercholesterolemia, arterial hypertension, cardiac remodeling, and rarefaction of the microcirculation in the heart and skeletal muscle.
- Subjects
METABOLIC syndrome; HIGH-fat diet; LABORATORY rats; LOW density lipoproteins; SYSTOLIC blood pressure; HYPERCHOLESTEREMIA; HYPERTRIGLYCERIDEMIA
- Publication
Obesity (19307381), 2013, Vol 21, Issue 10, p2046
- ISSN
1930-7381
- Publication type
Article
- DOI
10.1002/oby.20358