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- Title
Expression and regulation effects of chemokine receptor 7 in colon cancer cells.
- Authors
Li, Xiang; Wang, Xuemei; Li, Zitao; Liu, Yanjun; Sang, Liang; Zhang, Zhen; Zhang, Yixia
- Abstract
In China the incidence and mortality rates of colon cancer have been increasing annually. Studies have revealed that CXCR7 is expressed in many tumors. The aim of the present study was to investigate the function of CXCR7 in colon cancer. The expression level of chemokine receptor 7 (CXCR7) in Caco-2 and HCT116 cells was investigated to elucidate the effect of CXCR7 on cell biological behavior. Reverse transcription-quantitative PCR and western blot analysis were used to detect the expression level of CXCR7 in Caco-2 and HCT116 cells after transfection with small interfering (si)RNA. To analyze the in vitro biological function of CXCR7, cell proliferation was measured using a Cell Counting Kit-8 assay, and cell invasion and migration were measured using Matrigel, and Transwell and wound healing assays. siRNAs were successfully transfected into Caco-2 and HCT116 cells and resulted in a decrease in CXCR7 protein and mRNA expression. Downregulation of CXCR7 inhibited Caco-2 and HCT116 cell proliferation, invasion, and migration. Regulation of CXCR7 expression may affect the biological behavior of Caco-2 and HCT116 cells, suggesting that CXCR7 has a potential role in molecular therapy in colon cancer.
- Subjects
CHINA; CHEMOKINE receptors; COLON cancer; CANCER cells; WESTERN immunoblotting; CELL migration; SMALL interfering RNA
- Publication
Oncology Letters, 2020, Vol 20, Issue 1, p226
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2020.11561