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- Title
Pathways of apoptosis in human autosomal recessive and autosomal dominant polycystic kidney diseases.
- Authors
Goilav, Beatrice; Satlin, Lisa M.; Wilson, Patricia D.
- Abstract
Autosomal dominant polycystic kidney disease (ADPKD) is a major cause of end-stage renal disease in adults. Autosomal recessive (AR) PKD affects ∼1:20,000 live-born children with high perinatal mortality. Both diseases have abnormalities in epithelial proliferation, secretion, and cell–matrix interactions, leading to progressive cystic expansion and associated interstitial fibrosis. Cell number in a kidney reflects the balance between proliferation and apoptosis. Apoptosis results from extrinsic (ligand-induced, expression of caspase-8) and intrinsic (mitochondrial damage, expression of caspase-9) triggers. Previous studies have suggested a role for apoptosis in PKD cyst formation and parenchymal destruction. Mechanisms underlying apoptosis in human ADPKD and ARPKD were examined by quantitative immunohistochemistry and Western immunoblot analyses of age-matched normal and PKD tissues. Caspase-8 expression was significantly greater in small cysts and normal-appearing tubules than in larger cysts in ADPKD kidneys. Caspase-8 also appeared early in the disease process of ADPKD. In ARPKD, expression of caspase-8 was most pronounced in later stages of the disease and was not confined to a specific cyst size. In conclusion, apoptosis in human ADPKD is an early event, occurring predominantly in normal-appearing tubules and small cysts, and is triggered by an extrinsic factor, but it occurs later in ARPKD.
- Subjects
KIDNEY diseases in old age; POLYCYSTIC kidney disease; APOPTOSIS; CHRONIC kidney failure; CARCINOMA; IMMUNOHISTOCHEMISTRY; KIDNEY disease treatments; DISEASE risk factors
- Publication
Pediatric Nephrology, 2008, Vol 23, Issue 9, p1473
- ISSN
0931-041X
- Publication type
Article
- DOI
10.1007/s00467-008-0851-9