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- Title
A promiscuous cytochrome P450 aromatic Odemethylase for lignin bioconversion.
- Authors
Mallinson, Sam J. B.; Machovina, Melodie M.; Silveira, Rodrigo L.; Garcia-Borràs, Marc; Gallup, Nathan; Johnson, Christopher W.; Allen, Mark D.; Skaf, Munir S.; Crowley, Michael F.; Neidle, Ellen L.; Houk, Kendall N.; Beckham, Gregg T.; DuBois, Jennifer L.; McGeehan, John E.
- Abstract
Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.
- Subjects
CYTOCHROME P-450; LIGNINS; BIOCONVERSION; AROMATIC compounds; MONOMERS; GUAIACOL
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-04878-2