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- Title
miR-424 targets AKT3 and PSAT1 and has a tumor-suppressive role in human colorectal cancer.
- Authors
Fang, Yifeng; Liang, Xiao; Xu, Junfen; Cai, Xiujun
- Abstract
Background: Recent advances in cancer biology have uncovered critical roles for microRNAs in regulating tumor responses. This study is to elucidate the role of miR-424 in colorectal cancer development. Materials and methods: miR-424 expression was analyzed by qRT-PCR. The role of miR-424 was studied in cell lines and animal models. The downstream targets of miR-424 were determined by microarray analysis. Results: We found that miR-424 expression was downregulated in human colorectal cancer cell lines and patient biopsies. We demonstrated that miR-424 functioned as a tumor suppressor by suppressing colorectal cancer growth in vitro and in vivo and enhancing apoptosis. Using microarray screening, we subsequently presented evidence that miR-424 directly targeted the 3′ untranslated regions of the AKT serine/threonine kinase 3 (AKT3) and phosphoserine aminotransferase 1 (PSAT1) mRNAs via luciferase assay. Furthermore, AKT3 or PSAT1 silencing partially recapitulated the effects of miR-424. Conclusion: This newly identified miR-424/AKT3–SAT1 axis may represent a novel therapeutic strategy for future treatment of colorectal cancer.
- Subjects
COLORECTAL cancer; CELLS; CELL lines
- Publication
Cancer Management & Research, 2018, Vol 10, p6537
- ISSN
1179-1322
- Publication type
Article
- DOI
10.2147/CMAR.S185789