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- Title
FRECUENCIA OF Escherichia coli Y PATOTIPOS DE E. coli DIARREOGÉNICOS EN VEGETALES LISTOS PARA SU CONSUMO.
- Authors
DE LA O., José PINEDA; CORTÉS CUETO, Ana Laura; MONTES, Nancy LEÓN; SALAS RANGEL, Laura Patricia; GARCÍA REYES, Rocío Liliana; HELGUERA REPETTO, Addy Cecilia; RIVERA GUTIÉRREZ, Sandra; FERNÁNDEZ RENDÓN, Elizabeth; GONZÁLEZ MERCHAND, Jorge Alberto; CERNA CORTÉS, Jorge Francisco
- Abstract
Background: Diarrheagenic Escherichia coli pathotypes (DEP) are important foodborne pathogens and are classified according to their virulence traits: enteropathogenic E. coli (EPEC); enterotoxigenic E. coli (ETEC); Shiga toxin-producing E. coli (STEC); enteroinvasive E. coli (EIEC); enteroaggregative E. coli (EAEC) and diffuse adherent E. coli (DAEC). Recent DEP outbreaks have been associated with a wide variety of vegetables. Objectives: The aim of this study was to evaluate the presence of E. coli and DEP in ready-to-eat (RTE)-salads and RTE-sprouts collected in Mexico City. Methods: A total of 100 RTE-salads samples of raw vegetables were collected: 50 samples from different supermarkets (SPM) and 50 from street-vendor stalls (SVS). Also 100 RTE-sprouts samples were purchased: 50 from different SPM and 50 from SVS. E. coli strains were isolated by two methods: direct and indirect (from fecal coliforms). A total of 169 E. coli strains were analyzed by 3 multiplex PCR for identification of 11 DEP loci. Results: E. coli was detected in 34 (34%) samples of RTE-salads: 10 (20%) from SPM and 24 (48%) from SVS (p<0.05). A total of 23 (23%) of RTE-salads did not comply with the guideline. Five RTE-salads harbored DEP: four ETEC and one EAEC. For RTE-sprouts, 40 (40%) samples harbored E. coli strains: 18 (36%) from SPM and 22 (44%) from SVS. The best method for E. coli isolation from RTE-sprouts was the direct. Four (4%) RTE-sprouts harbored DEP: one ETEC, two STEC and one DAEC. Conclusions: The results of this study show that RTE-salads and RTE-sprouts harbored DEP, therefore, these foods are a potential risk for human illness. Measures to diminish or eliminate DEP from these food items might be advisable, such as a proper handling and washing before consumption of these products.
- Publication
Vitae (01214004), 2016, Vol 23, pS58
- ISSN
0121-4004
- Publication type
Article