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- Title
ACTIVIDAD BACTERICIDA IN VITRO DE Bacillus megaterium Y Lactococcus lactis CON ACTIVIDAD PROBIÓTICA CONTRA Aeromonas veronii Y Streptococcus agalactiae.
- Authors
GUTIÉRREZ RAMÍREZ, Luz Adriana; David RUALES, C. A.; ROMERO, Magally
- Abstract
Background: Pathogenic microorganisms as Aeromona sp. and Streptococcus agalactiae, has been produced big losses in the aquaculture industry, especially by the virulent species, an alternative to control the effect of these pathogens is through the use of probiotic micro-organisms. Objectives: In this research evaluated the bactericidal effect by the diffusion assay in wells, two extracts of bacteria Lactococcus lactis and Bacillus megaterium, previously isolated from intestine of tilapia (Oreochromis sp) against A.veronii and S.agalactiae. Methods: it was evaluated the antibacterial effect of extracts of L.lactis and Bacillus megaterium against A.veronii and S.agalactiae by the diffusion assay in wells, to determine the action of these, the diameter of the inhibition halos Summary, all areas of inhibition were greater than 5 mm. To determine the nature of the extracts of Bacillus megaterium were evaluated by spectrophotometry of mass and L.lactis by HPLC. Results: The results showed that both extracts controlled the growth of A.veronii and S.agalactiae, the statistical analysis did not show significant differences (p >0.05) in the inhibition generated between the two extracts of probiotics. Results by HPLC showed presence of lactic acid, while obtained in mass analyses showed the presence of acid palmitic, acid stearic and imidazole. Conclusions: All these findings demonstrate the positive effect of the probiotic microorganisms in the control of the growth of pathogens in the aquaculture industry.
- Publication
Vitae (01214004), 2016, Vol 23, pS24
- ISSN
0121-4004
- Publication type
Article