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- Title
Corosolic acid ameliorates cardiac hypertrophy via regulating autophagy.
- Authors
Zhao-Peng Wang; Difei Shen; Yan Che; Ya-Ge Jin; Sha-Sha Wang; Qing-Qing Wu; Heng Zhou; Yan-Yan Meng; Yuan Yuan
- Abstract
Aim: In this work, we explored the role of corosolic acid (CRA) during pressure overload-induced cardiac hypertrophy. Methods and results: Cardiac hypertrophy was induced in mice by aortic banding. Four weeks post-surgery, CRA-treated mice developed blunted cardiac hypertrophy, fibrosis, and dysfunction, and showed increased LC3 II and p-AMPK expression. In line with the in vivo studies, CRA also inhibited the hypertrophic response induced by PE stimulation accompanying with increased LC3 II and p-AMPK expression. It was also found that CRA blunted cardiomyocyte hypertrophy and promoted autophagy in Angiotensin II (Ang II)-treated H9c2 cells. Moreover, to further verify whether CRA inhibits cardiac hypertrophy by the activation of autophagy, blockade of autophagy was achieved by CQ (an inhibitor of the fusion between autophagosomes and lysosomes) or 3-MA (an inhibitor of autophagosome formation). It was found that autophagy inhibition counteracts the protective effect of CRA on cardiac hypertrophy. Interestingly, AMPK knockdown with AMPKa2 siRNA-counteracted LC3 II expression increase and the hypertrophic response inhibition caused by CRA in PE-treated H9c2 cells. Conclusion: These results suggest that CRA may protect against cardiac hypertrophy through regulating AMPK-dependent autophagy.
- Subjects
CARDIAC hypertrophy; ANGIOTENSIN II; LYSOSOMES; AUTOPHAGY; RESPONSE inhibition; IN vivo studies
- Publication
Bioscience Reports, 2019, Vol 39, Issue 12, p1
- ISSN
0144-8463
- Publication type
Article
- DOI
10.1042/BSR20191860