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- Title
Proteomics of the wheat tan spot pathogen Pyrenophora tritici‑repentis.
- Authors
Moffat, Caroline S.; Stoll, Thomas; Moolhuijzen, Paula
- Abstract
Objectives: The fungus Pyrenophora tritici-repentis is a major pathogen of wheat worldwide, causing the leaf spotting disease tan spot. To best inform approaches for plant genetic resistance, an understanding of the biology and pathogenicity mechanisms of this fungal pathogen is essential. Here, intracellular and extracellular proteins of P. triticirepentis were extracted, and peptides analysed via high-resolution mass spectrometry. Our objective was to generate a useful proteomics resource for P. tritici-repentis. A survey of proteins secreted by the pathogen into culture filtrate is especially useful, as these are likely to come in direct contact with the wheat host and may play important roles in infection/pathogenicity. The peptide data presented herein, has also been used to successfully verify and refine in silico predicted P. tritici-repentis gene annotations, through the validation of alternative splicing and reading frame shifts. Data description: The data sets presented consist of peptide spectra of the extracellular and intracellular proteomes of three P. tritici-repentis isolates. Peptide matches to translated transcripts of the North American reference isolate Pt- 1C-BFP are also provided.
- Subjects
PROTEOMICS; WHEAT tan spot; PYRENOPHORA; MICROBIAL virulence; SPECTROMETRY
- Publication
BMC Research Notes, 2018, Vol 11, Issue 1, p1
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/s13104-018-3936-y