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- Title
Real-Time Polymerase Chain Reaction Testing for Quantitative Evaluation of hOCT1 and MDR1 Expression in Patients with Chronic Myeloid Leukemia Resistant to Imatinib.
- Authors
Saeed Solali; Saeed Kaviani; Ali Akbar Movassaghpour; Mohammad Reza Aliparasti
- Abstract
Objective: To study quantitative expression of the genes MDR1 and hOCT1 in 30 patients with chronic myeloid leukemia (CML) and 27 healthy subjects. Methods: Total RNA was extracted from peripheral blood mononuclear cells. Complementary DNAs (cDNAs) were synthesized and realtime polymerase chain reaction (PCR) was performed. To evaluate the specificity of PCR products, melting curve analyses and gel electrophoresis were performed. Results: Our study results revealed an increased expression of MDR1 in CML patients compared to healthy controls. Patients with disease in the accelerated phase (AP) and in blastic crisis (BC) displayed higher expression of MDR1 compared with patients whose disease was in the chronic phase (CP).However, there was no significant difference in hOCT1-gene expression among CML patients and healthy controls. Conclusion: Increased expression of MDR1 can reduce the intracellular concentration of imatinib inside leukemic cells, resulting in the development of resistance to this drug. However, hOCT1 may play no significant role in the susceptibility of leukemic cells to imatinib in CML.
- Subjects
DRUG therapy; IMATINIB; CHRONIC myeloid leukemia; CELLULAR signal transduction; DRUG resistance in cancer cells; GENE expression; LONGITUDINAL method; PHOSPHOTRANSFERASES; POLYMERASE chain reaction; PROBABILITY theory; U-statistics; GENE expression profiling; DESCRIPTIVE statistics; GENETICS
- Publication
Laboratory Medicine, 2013, Vol 44, Issue 1, p13
- ISSN
0007-5027
- Publication type
Article
- DOI
10.1309/LMP1ECAE30JSVZEP