We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Hypomethylation of the promoter of the catalytic subunit of protein phosphatase 2A in response to hyperglycemia.
- Authors
Tros, Fabiola; Meirhaeghe, Aline; Hadjadj, Samy; Amouyel, Philippe; Bougnères, Pierre; Fradin, Delphine
- Abstract
In order to identify epigenetic mechanisms through which hyperglycemia can affect gene expression durably in β cells, we screened DNA methylation changes induced by high glucose concentrations (25 mmol/L) in the BTC3 murine cell line, using an epigenome-wide approach. Exposure of BTC3 cells to high glucose modified the expression of 1612 transcripts while inducing significant methylation changes in 173 regions. Among these 173 glucose-sensitive differentially methylated regions ( DMRs), 14 were associated with changes in gene expression, suggesting an epigenetic effect of high glucose on gene transcription at these loci. Among these 14 DMRs, we selected for further study Pp2ac, a gene previously suspected to play a role in β-cell physiology and type 2 diabetes. Using RT-q PCR and bisulfite pyrosequencing, we confirmed our previous observations in BTC3 cells and found that this gene was significantly demethylated in the whole blood cells (WBCs) of type 2 diabetic patients compared to controls.
- Subjects
DNA methylation; PHOSPHOPROTEIN phosphatases; HYPERGLYCEMIA; GENE expression; TYPE 2 diabetes; POLYMERASE chain reaction; BLOOD cells
- Publication
Physiological Reports, 2014, Vol 2, Issue 7, pn/a
- ISSN
2051-817X
- Publication type
Article
- DOI
10.14814/phy2.12076