We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lipase-Catalyzed Synthesis and Bioactivity of Capsinoids.
- Authors
DONG Xin-rong; LIU Zhong-hua; LI Yu-hong; XIE Da-ping
- Abstract
Capsinoids was chemoezymatically synthesised from capsaicinoids and their activities on PPARγ and cancer cell lines were studied. Immobilized lipase Novo 435 (1 g) was added to 100 mL dried acetone solution containing 50 mmol/L vanillyl alcohol,75 mmol/L methyl carboxylate and the mixture in a temperature-controlled incubator shaker at 30 °C was shaken for 24 h at 200 r/min. The product was obtained in 63% yield after being purified by silica gel chromatography. The product was composed of capsiate, dihydrocapsiate, nordihydrocapsiate and homodihydrocapsiate determined by GC-MS. Capsiate and dihydrocapsiate were separated by HPLC and characterized by ¹H NMR and EI-MS data. Bioscreening suggested that capsinoids are PPARγ agonists and inhibited the growth of MCF-7 and HepG2 cell lines at high concentration.
- Subjects
LIPASES; ORGANIC synthesis; PEROXISOMES; CELL lines; CANCER cells; SILICA gel; CHROMATOGRAPHIC analysis
- Publication
Natural Product Research & Development, 2009, Vol 21, Issue 4, p570
- ISSN
1001-6880
- Publication type
Article