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- Title
Hypothetical protein MAA_07646 is required for stress resistance and pathogenicity in Metarhizium robertsii.
- Authors
Chen, MingYue; Yu, YaShuai; Tong, YouMin; Wu, Hao; Qu, JiaoJiao; Yang, Yang; Huang, Bo
- Abstract
Metarhizium robertsii, a vital entomopathogenic fungus for pest management, relies on various virulence-related proteins for infection. Identifying these proteins, especially those with unknown functions, can illuminate the fungus's virulence mechanisms. Through RNA-seq, we discovered that the hypothetical protein MAA_07646 was significantly upregulated during appressorium formation in M. robertsii. In this study, we characterized MAA_07646, finding its presence in both the nucleus and cytoplasm. Surprisingly, it did not affect vegetative growth, conidiation, or chemical tolerance. However, it played a role in heat and UV radiation sensitivity. Notably, ΔMAA_07646 exhibited reduced virulence in Galleria mellonella larvae due to impaired appressorium formation and decreased expression of virulence-related genes. In conclusion, MAA_07646 contributes to thermotolerance, UV resistance, and virulence in M. robertsii. Understanding its function sheds light on the insecticidal potential of M. robertsii's hypothetical proteins.
- Subjects
METARHIZIUM; GREATER wax moth; FUNGAL virulence; HEAT radiation &; absorption; ENTOMOPATHOGENIC fungi; INSECT nematodes; RICE blast disease
- Publication
World Journal of Microbiology & Biotechnology, 2024, Vol 40, Issue 5, p1
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-024-03934-y