We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Inhibitory Effect of Chrysanthemum zawadskii Herbich var. latilobum Kitamura Extract on RANKL-Induced Osteoclast Differentiation.
- Authors
Dong Ryun Gu; Jin-Ki Hwang; Munkhsoyol Erkhembaatar; Kang-Beom Kwon; Min Seuk Kim; Young-Rae Lee; Seoung Hoon Lee
- Abstract
Chrysanthemum zawadskii Herbich var. latilobum Kitamura, known as "Gujulcho" in Korea, has been used in traditional medicine to treat various inflammatory diseases, including rheumatoid arthritis. However, these effects have not been tested on osteoclasts, the bone resorbing cells that regulate bone metabolism. Here, we investigated the effects of C zawadskii Herbich var. latilobum Kitamura ethanol extract (CZE) on osteoclast differentiation induced by treatment with the receptor activator of NF-kB ligand (RANKL). CZE inhibited osteoclast differentiation and formation in a dose-dependent manner. The inhibitory effect of CZE on osteoclastogenesis was due to the suppression of ERK activation and the ablation of RANKL-stimulated Ca2+-oscillation via the inactivation of PLCγ2, followed by the inhibition of CREB activation. These inhibitory effects of CZE resulted in a significant repression of c-Fos expression and a subsequent reduction of NFATcl, a key transcription factor for osteoclast differentiation, fusion, and activation in vitro and in vivo. These results indicate that CZE negatively regulates osteoclast differentiation and may be a therapeutic candidate for the treatment of various bone diseases, such as postmenopausal osteoporosis, rheumatoid arthritis, and periodontitis.
- Subjects
SOUTH Korea; PHYTOTHERAPY; ACADEMIC medical centers; BONE diseases; ASIAN medicine; OSTEOPOROSIS; PERIODONTITIS; POLYMERASE chain reaction; RESEARCH funding; RHEUMATOID arthritis; T-test (Statistics); WESTERN immunoblotting; IN vitro studies
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2013, Vol 2013, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2013/509482