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- Title
Alterations in intracellular potassium concentration by HIV-1 and SIV Nef.
- Authors
Bongkun Choi; Fermin, Cesar D.; Comardelle, Alla M.; Haislip, Allyson M.; Voss, Thomas G.; Garry, Robert F.
- Abstract
Background: HIV-1 mediated perturbation of the plasma membrane can produce an alteration in the transmembrane gradients of cations and other small molecules leading to cell death. Several HIV-1 proteins have been shown to perturb membrane permeability and ion transport. Xenopus laevis oocytes have few functional endogenous ion channels, and have proven useful as a system to examine direct effects of exogenously added proteins on ion transport. Results: HIV-1 Nef induces alterations in the intracellular potassium concentration in CD4+ T-lymphoblastoid cells, but not intracellular pH. Two electrode voltage-clamp recording was used to determine that Nef did not form ion channel-like pores in Xenopus oocytes. Conclusion: These results suggest that HIV-1 Nef regulates intracellular ion concentrations indirectly, and may interact with membrane proteins such as ion channels to modify their electrical properties.
- Subjects
CELL transformation; POTASSIUM channels; HIV; SIMIAN viruses; CELL membranes; CELL death; ION channels; MEMBRANE proteins
- Publication
Virology Journal, 2008, Vol 5, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/1743-422X-5-60