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- Title
The origin and distribution of 'Kokubu'-type splice-site mutations of the MLO genes in tobacco varieties.
- Authors
Masao Arai; Tomoyuki Komatsu; Hisashi Udagawa; Tomoyuki Tajima; Seiki Sato
- Abstract
The Japanese domestic tobacco (Nicotiana tabacum L.) cultivar 'Kokubu' shows high powdery mildew resistance that is controlled by splice-site mutations of two MILDEW LOCUS O genes, NtMLO1 and NtMLO2. We investigated the existence of the same NtMLO1/2 splice mutations in the genomes of various tobacco varieties cultivated in Japan and other countries. In total, 14 Japanese domestic cultivars, which were mainly distributed in Kagoshima, had splice-site mutations in both NtMLO1 and NtMLO2. In addition, tobacco cultivars containing only the NtMLO1 splice-site mutation were found in various tobacco production areas in Japan, but no cultivars with only the NtMLO2 splice-site mutation were detected. Moreover, the NtMLO1 splice-site mutation was detected in native Asian, Oriental and cigar tobacco varieties. Consequently, we speculate that these powdery mildew-resistant tobacco cultivars were generated relative recently in the Kagoshima area when a spontaneous mutation occurred at the NtMLO2 splice site in a cultivar already containing the NtMLO1 splice-site mutation and that the NtMLO1 splice-site mutation occurred during the early period of tobacco seed dissemination from the Americas to Asia and Japan.
- Subjects
KAGOSHIMA-shi (Japan); JAPAN; SEED dispersal; POWDERY mildew diseases; TOBACCO; GENOMES; CIGARS
- Publication
Breeding Science, 2022, Vol 72, Issue 3, p248
- ISSN
1344-7610
- Publication type
Article
- DOI
10.1270/jsbbs.22001