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- Title
Biodistribution of an oncolytic adenovirus after intracranial injection in permissive animals: a comparative study of Syrian hamsters and cotton rats.
- Authors
Sonabend, A. M.; Ulasov, I. V.; Han, Y.; Rolle, C. E.; Nandi, S.; Cao, D.; Tyler, M. A.; Lesniak, M. S.
- Abstract
Conditionally replicative adenoviruses (CRAds) are often evaluated in mice; however, normal and cancerous mouse tissues are poorly permissive for human CRAds. As the cotton rat (CR) is a semipermissive animal and the Syrian hamster (SH) is a fully permissive model for adenoviral replication, we compared them in a single study following intracranial (i.c.) injection of a novel glioma-targeting CRAd. Viral genomic copies were quantified by real-time PCR in brain, blood, liver and lung. The studies were corroborated by immunohistochemical, serological and immunological assays. CR had a multiple log higher susceptibility for adenoviral infection than SH. A similar amount of genomic copies of CRAd-Survivin-pk7 and human adenovirus serotype 5 (AdWT) was found in the brain of CR and in all organs from SH. In blood and lung of CR, AdWT had more genomic copies than CRAd-Survivin-pk7 in some of the time points studied. Viral antigens were confirmed in brain slices, an elevation of serum transaminases was observed in both models, and an increase in anti-adenoviral antibodies was detected in SH sera. In conclusion, CR represents a sensitive model for studying biodistribution of CRAds after i.c. delivery, allowing for the detection of differences in the replication of CRAd-Survivin-pk7 and AdWT that were not evident in SH.Cancer Gene Therapy (2009) 16, 362–372; doi:10.1038/cgt.2008.80; published online 14 November 2008
- Subjects
ADENOVIRUSES; COTTON rats as laboratory animals; GOLDEN hamster; VIRAL genomes; VIRAL replication; GLIOMAS; ONCOLOGY
- Publication
Cancer Gene Therapy, 2009, Vol 16, Issue 4, p362
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/cgt.2008.80