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- Title
Radioprotection of the murine submandibular gland by isoproterenol: autoradiography study with<sup>3</sup>H-leucine.
- Authors
Aonuma, Mitsuhiro; Nasu, Masanori; Iwata, Hiroshi; Yosue, Takashi
- Abstract
Irradiation of the salivary glands results in the generation of free radicals from metal ions present in the secretory granules of acinar cells, a process that is believed to exacerbate radiation damage to the salivary glands. We therefore conducted a comparative investigation of radiation damage to the acinar cells of murine submaxillary glands in which granule secretion had been induced, and used autoradiography to visualize the pathological changes. Male BALB/c mice, at 8 weeks of age, were divided into four groups: a no-isoproterenol (IPR) and no-irradiation group (group I), a no-IPR, irradiated group (group II), an IPR, no-irradiation group (group III), and an IPR, irradiated group (group IV). Intraperitoneal injections of IPR were used, and 3?h later, the submaxillary region was irradiated with X-rays at a dose of 10?Gy. Three days after the irradiation,3H-leucine was administered, and submaxillary glands were removed at predetermined times. Thin sections were prepared, and light- and electron-microscope autoradiography was performed. The number of reduced silver particles per unit acinar cell area was determined by light-microscopic autoradiography, and the proportion of reduced silver particles in the rough endoplasmic reticulum, the Golgi apparatus, and secretion granules was determined by electron-microscopic autoradiography. The result indicated that the effects of the radiation on the secretory potential of the submaxillary glands were diminished in acinar cells with a higher secretory granule content.
- Subjects
ISOPROTERENOL; AUTORADIOGRAPHY; LEUCINE; SUBMANDIBULAR gland; IRRADIATION
- Publication
Odontology, 2004, Vol 92, Issue 1, p14
- ISSN
1618-1247
- Publication type
Article
- DOI
10.1007/s10266-004-0032-7