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- Title
Microbiota from Rhabditis regina may alter nematode entomopathogenicity.
- Authors
Jiménez-Cortés, Jesús; Canales-Lazcano, Jorge; Lara-Reyes, Nancy; Rosenblueth, Mónica; Martínez-Romero, Esperanza; Contreras-Garduño, Jorge
- Abstract
Here we report the presence of the entomopathogenic nematode Rhabditis ( Rhabditoides) regina affecting white grubs ( Phyllophaga sp. and Anomala sp.) in Mexico and R. regina-associated bacteria. Bioassays were performed to test the entomopathogenic capacity of dauer and L2 and L3 (combined) larval stages. Furthermore, we determined the diversity of bacteria from laboratory nematodes cultivated for 2 years (dauer and L2-L3 larvae) and from field nematodes (dauer and L2-L3 larvae) in addition to the virulence in Galleria mellonella larvae of some bacterial species from both laboratory and field nematodes. Dauer and non-dauer larvae of R. regina killed G. mellonella. Bacteria such as Serratia sp. (isolated from field nematodes) and Klebsiella sp. (isolated from larvae of laboratory and field nematodes) may explain R. regina entomopathogenic capabilities. Different bacteria were found in nematodes after subculturing in the laboratory suggesting that R. regina may acquire bacteria in different environments. However, there were some consistently found bacteria from laboratory and field nematodes such as Pseudochrobactrum sp., Comamonas sp., Alcaligenes sp., Klebsiella sp., Acinetobacter sp., and Leucobacter sp. that may constitute the nematode microbiome. Results showed that some bacteria contributing to entomopathogenicity may be lost in the laboratory representing a disadvantage when nematodes are cultivated to be used for biological control.
- Subjects
NEMATODE infections; RHABDITIS; INSECT nematodes; GREATER wax moth; VIRULENCE of bacteria; MOLECULAR parasitology
- Publication
Parasitology Research, 2016, Vol 115, Issue 11, p4153
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-016-5190-3