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- Title
Cervine genotype is the major Cryptosporidium genotype in sheep in China.
- Authors
Yongli Wang; Yaoyu Feng; Bin Cui; Fuchun Jian; Changshen Ning; Rongjun Wang; Longxian Zhang; Lihua Xiao
- Abstract
Abstract  To identify Cryptosporidium species/genotypes in sheep in China and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in Henan Province (central China) were examined. Eighty-two Cryptosporidium-positive samples were analyzed by polymerase chain reaction (PCR)ârestriction fragment length polymorphism analysis of the small subunit (SSU) rRNA gene and PCR analysis of the 60 kDa glycoprotein (gp60) gene, and 41 were further analyzed by DNA sequencing of the PCR products. The SSU rRNA-based PCR identified two Cryptosporidium species and one genotype, including the Cryptosporidium cervine genotype (74/82), Cryptosporidium andersoni (4/82), and Cryptosporidium xiaoi (4/82). The cervine genotype was found in all age groups, C. xiaoi in lambs, and C. andersoni in ewes. There were intragenetic differences in the SSU rRNA gene sequences of the Cryptosporidium cervine genotype and C. xiaoi. No Cryptosporidium parvum was detected by both SSU rRNA- and gp60-based PCR assays. These findings suggest that sheep are a potential source for zoonotic infections of the Cryptosporidium cervine genotype.
- Subjects
HENAN Sheng (China); CHINA; SHEEP parasites; CRYPTOSPORIDIUM; POLYMERASE chain reaction; CRYPTOSPORIDIOSIS; RESTRICTION fragment length polymorphisms; P-glycoprotein; RNA; INFECTIOUS disease transmission
- Publication
Parasitology Research, 2010, Vol 106, Issue 2, p341
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-009-1664-x