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- Title
Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model.
- Authors
Wang, Dandan; Zhang, Huayong; Liang, Jun; Gu, Zhifeng; Ma, Xiaolei; Huang, Jing; Lin, Jing; Hou, Yayi; Lu, Liwei; Sun, Lingyun
- Abstract
Primary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4Foxp3 regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients.
- Subjects
HOMOGRAFTS; BONE marrow; MESENCHYMAL stem cells; STEM cell transplantation; CIRRHOSIS of the liver; LABORATORY mice; AUTOIMMUNE diseases; AMINOTRANSFERASES; CYTOKINES; T cells
- Publication
Clinical & Experimental Medicine, 2011, Vol 11, Issue 1, p25
- ISSN
1591-8890
- Publication type
Article
- DOI
10.1007/s10238-010-0105-6