We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Organelle antioxidants improve microspore embryogenesis in wheat and triticale.
- Authors
Asif, Muhammad; Eudes, François; Goyal, Aakash; Amundsen, Eric; Randhawa, Harpinder; Spaner, Dean
- Abstract
Low frequency of green plant production and albinism limits the use of isolated microspore culture (IMC) in cereal breeding programs. The present study was conducted in triticale and bread wheat IMC to increase the production of green plants and minimize albinism. NPB-99 + 10% Ficoll induction medium was supplemented with mitochondrial or plastid antioxidants, in a completely random design, to evaluate their contribution to successful microspore embryogenesis and green plant production. Each group of antioxidants was tested independently: first in triticale and then validated in various spring wheat genotypes. While the response differed by wheat genotype, induction medium supplemented with proline (10 mM) yielded a greater number of embryos/embryo-like structures and green plants in both triticale and wheat. No differences were found with respect to albinism in triticale or wheat except for the cv. Sadash. Among plastid antioxidants tested, glutathione (2 μM) proved to be the best antioxidant to increase embryo and green plant production. Salicylic acid also helped to reduce the number of albino plants in triticale and the wheat genotype SWS366. Overall, induction medium supplemented with proline or glutathione enhanced microspore embryogenesis in both triticale and wheat and increased the number of green plants in the recalcitrant genotypes.
- Subjects
ANTIOXIDANTS; MICROSPORES (Botany); SOMATIC embryogenesis; WHEAT; TRITICALE; PLANT productivity; ALBINOS &; albinism; PLANT cell culture
- Publication
In Vitro Cellular & Developmental Biology Plant, 2013, Vol 49, Issue 5, p489
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-013-9514-z