We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Rapid detection of extended-spectrum {beta}-lactamase-producing Gram-negative bacilli in blood cultures.
- Authors
Sarjana Jain; Jenny Andrews; Adam Fraise; Nigel Brenwald
- Abstract
Objectives Conventional detection of extended-spectrum β-lactamase (ESBL) producers in blood cultures usually requires overnight incubation. This could delay the prescribing of appropriate therapy. We evaluated whether the chromogenic cephalosporin HMRZ-86, which is hydrolysed by ESBLs, could be used for the rapid detection of ESBL producers directly in blood culture broths. Methods The HMRZ-86 test was first applied to broth cultures of isolates producing known β-lactamases. A colour change indicating hydrolysis, which was inhibited by clavulanic acid, was taken as an indication of ESBL production. A similar method was used for testing blood culture supernatants and broth subcultures of blood cultures. Results The HMRZ-86 test detected all the ESBL producers among 83 clinical isolates and control strains. Only one false positive was seen. The usefulness of HMRZ-86 for the direct testing of blood culture broths was limited by the presence of lysed blood. However, by using a 2 h broth subculture of the blood culture broths, the HMRZ-86 test was able to detect all those blood cultures containing an ESBL producer. No false positive or negative tests occurred according to the results of our standard phenotypic tests. Conclusions The HMRZ-86 test is a simple and rapid method that can be used for detecting ESBL producers in blood cultures.
- Subjects
HYDROLYSIS; MICROBIAL enzymes; CHROMOGENIC compounds; BETA lactam antibiotics
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2007, Vol 60, Issue 3, p652
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkm256