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- Title
Complete genome sequence of pueraria virus A, a new member of the genus Caulimovirus.
- Authors
Gudeta, Workitu Firomsa; Igori, Davaajargal; Belete, Mesele Tilahun; Kim, Se Eun; Moon, Jae Sun
- Abstract
The complete genome sequence of a new caulimovirus in Pueraria montana was determined using high-throughput sequencing. The 7,572 nucleotide genome of pueraria virus A (PVA) contains genes that encode a movement protein, an aphid transmission factor, a virion-associated protein, a coat protein, a protease + reverse transcriptase + ribonuclease H, and a transactivator/viroplasmin protein, as well as two intergenic regions, which are all common features of members of the genus Caulimovirus. A sequence alignment revealed that the complete genome of PVA shares 66.82% nucleotide sequence identity with strawberry vein banding virus (GenBank accession no. KX249738.1). The results of phylogenetic analysis and the observation that the nucleotide sequence of the polymerase coding region differed by more than 20% indicated that PVA is a member of a new species the genus Caulimovirus, family Caulimoviridae.
- Subjects
WHOLE genome sequencing; COAT proteins (Viruses); RIBONUCLEASE H; NUCLEOTIDE sequence; PUERARIA; PLASMIN; REVERSE transcriptase; NUCLEOTIDE sequencing
- Publication
Archives of Virology, 2022, Vol 167, Issue 6, p1481
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-022-05431-9