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- Title
Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip.
- Authors
Yang, Bo; Huang, Jiaxin; Tao, Chunxian; Li, Zhenqing; Zhang, Dawei; Yamaguchi, Yoshinori
- Abstract
Continuous-flow PCR (CF-PCR) can realize rapid DNA amplification because of the high temperature variation rate. However, off-line detection methods for PCR may induce cross contamination. To overcome this problem, we herein fabricated an integrated CF-PCR and electrophoresis microfluidic chip. The optimal voltage applied in the electrophoresis part of the microfluidic chip was achieved by simulation in COMSOL. Coating the inside wall of the microchannel can inhibit electroosmotic flow and improve the resolution for DNA fragments. The temperature distribution of the serpentine part can meet the PCR and has no obvious suppressive effect on sample separation. Finally, we have performed the amplification of target genes for Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola and detected the corresponding PCR products in the microfluidic chip within 11 min. Such work provides a new method for the rapid detection of bacteria.
- Subjects
CAPILLARY electrophoresis; ELECTRO-osmosis; CAPILLARY flow; GENE amplification; PORPHYROMONAS gingivalis; TEMPERATURE distribution; PATHOGENIC microorganisms
- Publication
Separations (2297-8739), 2023, Vol 10, Issue 4, p271
- ISSN
2297-8739
- Publication type
Article
- DOI
10.3390/separations10040271