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- Title
Effects of the Nocth signaling pathway on expression of inflammatory factors and transforming growth factors in diabetic foot ulcers.
- Authors
Qiang Han; Guo-Bin Liu; Ren-Yan Huang; Feng Xu; Shi-Meng Yan; Chen-Yan Shi; Jun-Hao Chen
- Abstract
Objective: persistent hyperinflammation is an important reason for the development of diabetic foot ulcer. Notch signaling is an important signaling pathway involved in the inflammatory response and cell proliferation in diabetic foot ulcer rats. This paper aims to explore the effect of Notch signaling on inflammatory factors, chemokines and growth factors through the intervention of Notch signaling in diabetic foot ulcer rats. Methods: the experimental model was made by using high-fat feed combined with streptozotocin (STZ) to cause diabetes, and the experimental model of diabetic foot ulcer was established by constant temperature and constant pressure scald apparatus. The normal ulcer model was used as a control. The intervention controls of the experimental model included normal saline, western medicine growth factor, Notch agonist Jagged1, Notch signaling inhibitor ly-411575, and the intervention of traditional Chinese medicine Zizhu ointment for 7 days. Serum il-1, il-6, TNF-radiation, and il-17 were detected by ELISA. Real-time PCR was used to detect the inflammatory factors, chemokines, and growth factors associated with Notch signaling in wound tissues: tnf-uum, il-1, il-6, il-17, interleukin-8, ip-10, McP-1, TGF- uum, TGF- livelihood. Results: serum levels of il-1, il-6, TNF- radiation and il-17 in diabetic foot ulcer rats were significantly higher than that in normal ulcer rats. The contents of il-1, il-6, TNF- radiation and il-17a in ly-411575 group and Zizhu ointment group were significantly reduced. Real-time PCR results of wound tissue showed that the levels of inflammatory cytokines il-1, il-6, TNF- radiation, il-17 and chemokines ip-10, il-8 and McP-1 in the wound tissue of diabetic foot ulcer rat model were significantly higher than that of normal ulcer model, and the levels of growth factor TGF- exposure were lower than that of normal ulcer model.LY-411575 significantly reduced il-1, il-6, TNF- maxima, il-17, and the chemokines ip-10, il-8, and McP-1 in diabetic foot ulcer rats, and reduced the expression of TGF-, TGF- earth. Jagged can increase the expression of TGF- -, TGF- - -, suggesting that inhibition of the Notch signaling pathway can reduce the expression of the inflammatory factors il-1, il-6, TNF- -, il-17a, il-8, and the growth factors TGF- -, TGF- - -. Zizhu ointment can reduce the levels of il-1, il-6, TNF- benand, il-17, and the chemokines ip-10, il-8, and McP-1 on the wound surface of diabetic foot rats, and improve the expression of TGF- benand TGF- SUNS.Ly-411575 inhibited the expression of TGF- bento and TGF- promoting of Zizhu ointment. Conclusion: the expression of inflammatory cytokines and chemokines was higher and the expression of growth factors was lower in diabetic foot ulcer rats than in normal ulcer rats. Inhibition of Notch signaling pathway can reduce the expression of inflammatory factors, chemokines and growth factors in experimental model rats, and Notch signaling pathway can promote inflammation and cell proliferation. Zizhu ointment can reduce the levels of inflammatory cytokines and chemokines in diabetic foot ulcer rats, improve the expression of growth factors, and reduce wound inflammation, which may be related to the inhibition of Nocth signal expression.
- Subjects
NOTCH signaling pathway; NOTCH genes; DIABETIC foot; INFLAMMATION; TRANSFORMING growth factors
- Publication
Journal of Hainan Medical University, 2021, Vol 27, Issue 12, p1
- ISSN
1007-1237
- Publication type
Article