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- Title
Age-Related Mitochondrial DNA Deletion in Rat Liver Depends on Dietary Fat Unsaturation.
- Authors
Quiles, José L.; Ochoa, Julio J.; Ramirez-Tortosa, M. Carmen; Huertas, Jesús R.; Mataix, José
- Abstract
We fed male Wistar rats lifelong on virgin olive (rich in the monounsaturated oleic acid) or sunflower (rich in the polyunsaturated linoleic acid) oil-based diets. At 6 and 24 months, liver mitochondria were analyzed for a mitochondrial DNA (mtDNA) deletion, reactive oxygen species, antioxidants, and ultrastructural alterations. An aging-related increase in the relative amount of the deletion was observed for both dietary groups, being higher in animals fed sunflower oil. Oxidative stress was lower in virgin olive oil-fed animals. Aging led to higher superoxide dismutase, catalase, and glutathione peroxidase activities and increased α-tocopherol and coenzyme Q. Mitochondria from aged animals fed sunflower oil exhibited a lower number of cristae and a higher circularity. Results suggest that the age-related increase of the relative amount of deleted mtDNA depends on fat unsaturation. Moreover, the studied mtDNA deletion was correlated with mitochondrial oxidative stress and ultrastructural alterations.
- Subjects
MITOCHONDRIA; OLEIC acid; MITOCHONDRIAL DNA; MONOUNSATURATED fatty acids; OXIDATIVE stress; AGING
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2006, Vol 61, Issue 2, p107
- ISSN
1079-5006
- Publication type
Article
- DOI
10.1093/gerona/61.2.107