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- Title
Molecular Diagnosis and Transmission Studies of Chilli Leaf Curl Virus by Asia-I Cryptic Species of Whitefly (Bemisia tabaci Genn.).
- Authors
MADHU KIRAN, GUNDA V. N. S.; NAGARAJU, N.; RAO, A. MOHAN
- Abstract
Chilli (Capsicum annuum L.)is major vegetable cum spice crop known to be infected with several begomoviruses resulting in severe yield losses. Chilli samples showing typical begomovirus symptoms were collected from farmer fields and assessed for detection using ChiLCV coat protein gene specific primers. The genome analysis showed the virus had 98.3 per cent nucleotide identity with Chilli leaf curl virus (ChiLCV). The whitefly samples collected from farmer's fields were identified as Asia-I cryptic species based on mtCO1 gene analysis. The point mutations in coat protein were identified and subjected to in silico analysis with bioinformatic tools to determine the effect of amino acid substitutions as deleterious or neutral as it influences the transmission characteristics of whitefly species. The interaction studies of ChiLCV with Asia-I cryptic species revealed that twenty whiteflies with 24 h of acquisition access period (AAP) and inoculation access period (IAP) each successfully transmitted the virus with 100.0 per cent transmission. Asia-I cryptic species females found to be more efficient transmitters (86.6%) of ChiLCV than males (53.0%). The susceptibility of chilli seedlings to leaf curl virus is inversely proportional with age. This study established the tripartite interaction relationship between ChiLCV, Asia-I cryptic species of whitefly and chilli host.
- Subjects
CAPSICUM annuum; PEPPER diseases &; pests; VEGETABLE diseases &; pests; SWEETPOTATO whitefly; BEMISIA
- Publication
Mysore Journal of Agricultural Sciences, 2021, Vol 55, Issue 4, p121
- ISSN
0047-8539
- Publication type
Article