We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Auxin-Cytokinin Cross Talk in Somatic Embryogenesis of Coffea canephora.
- Authors
Avilez-Montalvo, Johny R.; Quintana-Escobar, Ana O.; Méndez-Hernández, Hugo A.; Aguilar-Hernández, Víctor; Brito-Argáez, Ligia; Galaz-Ávalos, Rosa M.; Uc-Chuc, Miguel A.; Loyola-Vargas, Víctor M.
- Abstract
Cytokinins (CK) are plant growth regulators involved in multiple physiological processes in plants. One less studied aspect is CK homeostasis (HM). The primary genes related to HM are involved in biosynthesis (IPT), degradation (CKX), and signaling (ARR). This paper demonstrates the effect of auxin (Aux) and CK and their cross talk in a Coffea canephora embryogenic system. The transcriptome and RT-qPCR suggest that Aux in pre-treatment represses biosynthesis, degradation, and signal CK genes. However, in the induction, there is an increase of genes implicated in the CK perception/signal, indicating perhaps, as in other species, Aux is repressing CK, and CK are inducing per se genes involved in its HM. This is reflected in the endogenous concentration of CK; pharmacology experiments helped study the effect of each plant growth regulator in our SE system. We conclude that the Aux–CK balance is crucial to directing somatic embryogenesis in C. canephora.
- Subjects
COFFEE; SOMATIC embryogenesis; PLANT regulators; CYTOKININS
- Publication
Plants (2223-7747), 2022, Vol 11, Issue 15, p2013
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants11152013