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- Title
The Genetic Complexity of Type-IV Trichome Development Reveals the Steps towards an Insect-Resistant Tomato.
- Authors
Vendemiatti, Eloisa; Therezan, Rodrigo; Vicente, Mateus H.; Pinto, Maísa de Siqueira; Bergau, Nick; Yang, Lina; Bernardi, Walter Fernando; Alencar, Severino M. de; Zsögön, Agustin; Tissier, Alain; Benedito, Vagner A.; Peres, Lázaro E. P.
- Abstract
The leaves of the wild tomato Solanum galapagense harbor type-IV glandular trichomes (GT) that produce high levels of acylsugars (AS), conferring insect resistance. Conversely, domesticated tomatoes (S. lycopersicum) lack type-IV trichomes on the leaves of mature plants, preventing high AS production, thus rendering the plants more vulnerable to insect predation. We hypothesized that cultivated tomatoes engineered to harbor type-IV trichomes on the leaves of adult plants could be insect-resistant. We introgressed the genetic determinants controlling type-IV trichome development from S. galapagense into cv. Micro-Tom (MT) and created a line named "Galapagos-enhanced trichomes" (MT-Get). Mapping-by-sequencing revealed that five chromosomal regions of S. galapagense were present in MT-Get. Further genetic mapping showed that S. galapagense alleles in chromosomes 1, 2, and 3 were sufficient for the presence of type-IV trichomes on adult organs but at lower densities. Metabolic and gene expression analyses demonstrated that type-IV trichome density was not accompanied by the AS production and exudation in MT-Get. Although the plants produce a significant amount of acylsugars, those are still not enough to make them resistant to whiteflies. We demonstrate that type-IV glandular trichome development is insufficient for high AS accumulation. The results from our study provided additional insights into the steps necessary for breeding an insect-resistant tomato.
- Subjects
TOMATO breeding; TOMATOES; GENE mapping; TRICHOMES; FOLIAGE plants; GENE expression
- Publication
Plants (2223-7747), 2022, Vol 11, Issue 10, p1309
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants11101309