We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
BRE-AS启动子负调控区变异与启动子活性、母猪繁殖力的关系.
- Authors
王杨; 王苗苗; 单保森; 吴望军; 李齐发
- Abstract
[Objectives]This study was aimed to investigate the sequence characteristics of the promoter negative regulatory region (PNRR) of the porcine BRE antisense lncRNA (BRE-AS) gene, and the relationship between its variant polymorphisms and sow reproductive traits and promoter activity. [Methods]The PNRR sequence of BRE-AS in Yorkshire pigs was obtained by DNA sequencing. Variants in Yorkshire pig BRE-AS PNRR were screened by pooled-DNA sequencing, and genotyped by DNA sequencing. Association analysis of polymorphisms, haplotypes and sow reproductive traits was poformed by using GLM model. The effect of variants on promoter activity of BRE-AS was analyzed by using a luciferase reporter system. [Results]Porcine BRE-AS PNRR was located at-952--161 nt of promoter, with a length of 792 bp. Two variants were found at-794 nt and-756 nt of the BRE-AS PNRR in Yorkshire pigs, which were named as g.-794T>G and g.-756A>C respectively. For SNP g.-794T>G,three genotypes were detected in Yorkshire population, and TT was the dominant genotype (0.752) and T was the dominant allele (0.863) . For SNP g.-756A>C,three genotypes were also detected, and AA was the dominant genotype (0.759) and A was the dominant allele (0.868) . Two variants formed three combined genotypes, of which TT/AA was the dominant genotype (0.760) and T-A was the dominant haplotype (0.869) . Association analysis showed that the two SNP and the combined genotype were significantly (P<0.05 or P<0.01) associated with three reproductive traits, including total number of piglets born (TNB),number of piglets born alive (NBA) and number of healthy piglets (NSP) . Double-luciferase activity assays showed that activity of promoter with haplotype G-C was significantly higher than that of promoter with haplotype T-A (P<0.05) . [Conclusions]BRE-AS was a candidate gene for sow reproductive traits in Yorkshire pigs, and two variants (g.-794T>G and g.-756A>C) in the PNRR affected sow fertility by affecting the promoter activity.
- Subjects
YORKSHIRE swine
- Publication
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao, 2023, Vol 46, Issue 6, p1153
- ISSN
1000-2030
- Publication type
Article
- DOI
10.7685/jnau.202204002