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- Title
长角血蜱副肌球蛋白的原核表达、 纯化与免疫原性分析.
- Authors
崔雪娇; 曹园园; 李芬; 郑晶晶; 胡永红
- Abstract
In order to study the immune protection of Pmy from Haemaphysalis longicomis for next step* recombinant plasmids pET-32(a+)-pmy were transferred into E. coli BL21 for prokaryotic expression. The SDS-PAGE results show that rPmy contents of expression in supernatant reach a maximum when the optimum conditions are 0. 5 mmol/L of IPTG dose, 25 °C of induction temperature and 6 h of induction time. The rPmy was purified by affinity chromatography. Western blotting shows that rPmy has immunogenicity. This study provides previous research base for the development of vaccine against ticks.
- Publication
China Sciencepaper, 2017, Vol 12, Issue 12, p1418
- ISSN
2095-2783
- Publication type
Article