We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Peak Shapes and Their Measurements: The Need and the Concept Behind Total Peak Shape Analysis.
- Authors
Wahab, M. Farooq; Patel, Darshan C.; Armstrong, Daniel W.
- Abstract
Gaussian peak shapes in chromatography are indicative of a well-behaved system. Such peak shapes are highly desirable from the perspective of column packing technology. From an analyst's point of view, Gaussian peaks provide improved sensitivity (lower detection limits) and allow ease of quantitation. In practice, one can obtain peaks that tail, front, or concurrently front and tail for reasons such as column packing issues, chemical and kinetic effects, and suboptimal high performance liquid chromatography (HPLC) system plumbing and detector settings. Here, we discuss a number of approaches for peak shape measurement that are available in modern chromatography software, along with their advantages and drawbacks. A new "total peak shape analysis" approach is suggested that facilitates detection and quantification of concurrent fronting and tailing in peaks. Several remediation approaches are proposed that can help chromatographers analyze and improve peak shapes.
- Subjects
HIGH performance liquid chromatography; CHROMATOGRAPHIC analysis; FLUID dynamics; DIGITAL filters (Mathematics); THERMODYNAMICS
- Publication
LC-GC Europe, 2017, Vol 30, Issue 12, p670
- ISSN
1471-6577
- Publication type
Article