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- Title
Synergistic Effects of Azithromycin and STING Agonist Promote IFN-I Production by Enhancing the Activation of STING-TBK1 Signaling.
- Authors
Petcharat, Kanoktip; Munkong, Narongsuk; Thongboontho, Rungthip; Chartarrayawadee, Widsanusan; Thim-Uam, Arthid
- Abstract
Therefore, this study aims to evaluate the role of AZM and STING on IFN-I responses in macrophages.Methods: RAW 264.7 macrophages were treated with AZM with and without a STING-agonist (DMXAA), and the maturation of macrophages was determined using flow cytometry. Gene expression and pro-inflammatory cytokines were analyzed using qPCR and ELISA, respectively. Moreover, protein expression was investigated using Western blot assays and immunofluorescence.Results: Our results show that AZM significantly induced M1 phenotypes, promoting surface molecule expansion of CD80 and MHC-II and production of IL-6 and TNF-α cytokines on DMXAA-stimulated macrophages. Furthermore, we found that AZM-increased mRNA levels of interferon-stimulated genes (ISGs) could be due to the high expression of STNG-TBK1 signaling in the presence of DMXAA.Conclusion: Our data suggest that AZM enhancement of IFN-I responses was STING dependent in DMXAA-stimulated macrophages. These data underline a novel approach to AZM action-mediated STING-TBK1 signaling for regulating IFN-I responses and may further augment the scientific basis and potential use of AZM in clinical applications.
- Subjects
AZITHROMYCIN; GENE expression; TYPE I interferons; CLINICAL medicine; PROTEIN expression
- Publication
Journal of Experimental Pharmacology, 2023, Vol 15, p407
- ISSN
1179-1454
- Publication type
Article
- DOI
10.2147/JEP.S433181