We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-x<sub>L</sub> and promotes apoptosis.
- Authors
Komatsu, Kiyoshi; Miyashita, Toshiyuki; Hang, Haiying; Hopkins, Kevin M.; Zheng, Wei; Cuddeback, Sandy; Yamada, Masao; Lieberman, Howard B.; Wang, Hong-Gang
- Abstract
DNA damage induces apoptosis through a signalling pathway that can be suppressed by the BCL-2 protein, but the mechanism by which DNA damage does this is unknown. Here, using yeast two-hybrid and co-immunoprecipitation studies, we show that RAD9, a human protein involved in the control of a cell-cycle checkpoint, interacts with the antiapoptotic Bcl-2-family proteins BCL-2 and BCL-x[sub L], but not with the pro-apoptotic BAX and BAD. When overexpressed in mammalian cells, RAD9 induces apoptosis that can be blocked by BCL-2 or BCL-x[sub L]. Conversely, antisense RAD9 RNA suppresses cell death induced by methyl methanesulphonate. These findings indicate that RAD9 may have a new role in regulating apoptosis after DNA damage, in addition to its previously described checkpoint-control and other radioresistance-promoting functions.
- Subjects
SCHIZOSACCHAROMYCES pombe; DNA damage; PROTEINS
- Publication
Nature Cell Biology, 2000, Vol 2, Issue 1, p1
- ISSN
1465-7392
- Publication type
Article
- DOI
10.1038/71316